In the catalase test, the formation of bubbles is due to

In the catalase test, the formation of bubbles is due to

• A. Production of oxygen (O2)
• B. Production of hydrogen gas (H2)
• C. Breakdown of water
• D. Oxidation of hydrogen peroxide

Answer: (A)

The test shows whether catalase is present by watching for gas bubbles when hydrogen peroxide is added. Catalase rapidly decomposes hydrogen peroxide into water and oxygen. The bubbles come from oxygen gas being released into the solution as the reaction occurs: 2 H2O2 → 2 H2O + O2. So the formation of bubbles signifies production of oxygen gas, not hydrogen gas, not breakdown of water, and not an oxidation of hydrogen peroxide. This bubbling is the hallmark of a positive catalase result.

The test shows whether catalase is present by watching for gas bubbles when hydrogen peroxide is added. Catalase rapidly decomposes hydrogen peroxide into water and oxygen. The bubbles come from oxygen gas being released into the solution as the reaction occurs: 2 H2O2 → 2 H2O + O2. So the formation of bubbles signifies production of oxygen gas, not hydrogen gas, not breakdown of water, and not an oxidation of hydrogen peroxide. This bubbling is the hallmark of a positive catalase result.